Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 423, Issue C, Pages 96-112Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2016.01.001
Keywords
Testis; AMP-activated protein kinase; Sertoli cells; Fertility; Blood testis barrier; Metabolism
Categories
Funding
- national program FERTiNERGY - French National Research Agency (ANR)
- Region Centre
- Institut National de la Recherche Agronomique
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The AMP-activated protein kinase (AMPK) is an important regulator of cellular energy homeostasis which plays a role in fertility. Complete disruption of the AMPK catalytic subunit alpha 1 gene (alpha 1AMPK KO) in male mice results in a decrease in litter size which is associated with the production of altered sperm morphology and motility. Because of the importance of Sertoli cells in the formation of germ cells, we have chosen to selectively disrupt alpha 1AMPK only in the Sertoli cells in mice (Sc-alpha 1AMPK-KO mice). Specific deletion of the alpha 1AMPK gene in Sertoli cells resulted in a 25% reduction in male fertility associated with abnormal spermatozoa with a thin head. No clear alterations in testis morphology or modification in the number of Sertoli cells in vivo were observed, but a dysregulation in energy metabolism in Sertoli cells occurred. We have reported an increase in lactate production, in lipid droplets, and a reduction in ATP production in Sc-alpha 1AMPK-KO Sertoli cells. These perturbations were associated with lower expression of mitochondrial markers (cytochrome c and PGC1-alpha). In addition another metabolic sensor, the deacetylase SIRT1, had a reduction in expression which is correlated with a decline in deacetylase activity. Finally, expression and localization of junctions forming the blood-testis barrier between Sertoli cells themselves and with germ cells were deregulated in Sc-alpha 1AMPK-KO. In conclusion, these results suggest that dysregulation of the energy sensing machinery exclusively through disruption of alpha 1AMPK in Sertoli cells translates to a reduction in the quality of germ cells and fertility. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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