Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 417, Issue 1-2, Pages 35-47Publisher
SPRINGER
DOI: 10.1007/s11010-016-2711-4
Keywords
Apoptosis; Autophagy; CHOP; mTOR; NF-kappa B2; ORP150
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Funding
- Medical University of Bialystok [POPW.01.03.00-20-001/12]
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Cancer cells have developed a number of adaptation mechanisms involving the signal activation of the transduction pathways, which promotes the progression and metastasis. Our results showed that the percentage of apoptotic MCF-7 cells incubated in the low glucose medium for 48 h was lower in comparison to those cultured in the high glucose medium, despite the high expression of the proapoptotic transcription factor-CHOP. Furthermore, the MCF-7 cells incubated in the low glucose medium for 48 h showed a higher expression of NF-kappa B p100/p52 subunits compared to cells incubated in the high glucose medium. Moreover, our findings demonstrated that the shortage of glucose strongly induces autophagy in MCF-7 cells. The activation of this process is not associated with the changes in the expression of mTOR kinase. We suggest, that the antiapoptotic chaperone ORP150 induction, transcription factor NF-kappa B2 activation, and increased autophagy constitute mechanisms protecting the MCF-7 cells against apoptosis.
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