4.7 Article

Transfer of patient's peripheral blood mononuclear cells (PBMCs) disrupts blood-brain barrier and induces anti-NMDAR encephalitis: a study of novel humanized PBMC mouse model

Journal

JOURNAL OF NEUROINFLAMMATION
Volume 20, Issue 1, Pages -

Publisher

BMC
DOI: 10.1186/s12974-023-02844-4

Keywords

Humanized mouse model; Anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis; Peripheral blood mononuclear cells (PBMC); Blood-brain barrier (BBB); Il-1 ss; Anakinra

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In this study, a novel humanized mouse model of anti-NMDAR encephalitis was established by injecting patients' peripheral blood mononuclear cells into mice. The engraftment of patients' cells produced potent anti-GluN1 autoantibodies and disrupted the blood-brain barrier, leading to behavioral and cognitive changes in the mice. Il-1 ss was identified as a key gene involved in these pathological changes.
Background Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is a severe autoimmune neuropsychiatric disease. Brain access of anti-NMDAR autoantibody through the blood-brain barrier (BBB) is essential for pathogenesis. Most previous animal models limit the investigation of etiologies of BBB damage in patients. Methods In this study, we established a novel humanized mouse model of anti-NMDAR encephalitis by intraperitoneal injection of patients' peripheral blood mononuclear cells (PBMCs) into BALB/c Rag2(-/-)Il2rg(-/-)Sirpa(NOD)Flk2(-/-) mice. Results We found that engraftment of patients' PBMCs not only produced potent anti-GluN1 autoantibodies, but also disrupted BBB integrity to allow brain access of autoantibodies, resulting in a hyperactive locomotor phenotype, anxiety- and depressive-like behaviors, cognitive deficits, as well as functional changes in corresponding brain regions. Transcriptome analysis suggested an exaggerated immune response and impaired neurotransmission in the mouse model and highlighted Il-1 ss as a hub gene implicated in pathological changes. We further demonstrated that Il-1 ss was produced by endothelial cells and disrupted BBB by repressing tight junction proteins. Treatment with Anakinra, an Il-1 receptor antagonist, ameliorated BBB damage and neuropsychiatric behaviors. Conclusions Our study provided a novel and clinically more relevant humanized mouse model of anti-NMDAR encephalitis and revealed an intrinsic pathogenic property of the patient's lymphocytes.

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