Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 207, Issue 2, Pages 75-83Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2016.06.001
Keywords
SPARC; Excretory/secretory products; Strongyloides; Immune response; 3D-cell culture
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Funding
- Evangelisches Studienwerk Villigst
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The secreted protein acidic and rich in cysteine (SPARC), found in the excretory/secretory products of Strongyloides ratti, is most strongly expressed in parasitic females. Since SPARC proteins are involved in the modulation of cell-matrix interactions, a role of the secreted S. ratti SPARC (Sr-SPARC) in the manifestation of the parasite in the host's intestine is postulated. The full-length cDNA of Sr-SPARC was identified and the protein was recombinantly expressed. The purified protein was biologically active, able to bind calcium, and to attach to mucosa-associated human cells. Addition of Sr-SPARC to an in vitro mucosal three-dimensional-cell culture model led to a time-dependent release of the cytokines TNF-alpha, IL-22, IL-10 and TSLP. Of importance, exposure with Sr-SPARC fostered wound closure in an intestinal epithelial cell model. Here, we demonstrate for the first time that SPARC released from the nematode is a multifunctional protein affecting the mucosal immune system. (C) 2016 Elsevier B.V. All rights reserved.
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