Electrochemical sensor based on molecularly imprinted copolymer for selective and simultaneous determination of riboflavin, dopamine, and L-tryptophan

This research shows the exact detection of riboflavin (RF), dopamine (DA), and L-tryptophan (Trp) through molecularly imprinted polymer (MIP) based on the electropolymerization method. MIP was placed on the surface of the glassy carbon electrode (GCE) by electropolymerization of monomers such as catechol and para-aminophenol, in the presence of all three analytes. The introduced sensor was investigated using field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), Fourier-transform infrared spectroscopy (FTIR), and electrochemical methods, for example, electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV). The MIP/GCE performs well in terms of selectivity, reproducibility, repeatability, and stability. This sensor revealed good linear ranges of 0.005-500 mu M for RF, 0.05-500 mu M for DA, and 0.1-250 mu M for Trp with limits of detection (LOD) as 0.0016 mu M, 0.016 mu M, and 0.03 mu M for RF, DA, and Trp, respectively. The modified GCE was successfully applied to detect RF, DA, and Trp in serum and milk samples with satisfactory results.

Automated summary

This research developed a sensor based on molecularly imprinted polymer (MIP) for the precise detection of riboflavin (RF), dopamine (DA), and L-tryptophan (Trp) using the electropolymerization method. The MIP/GCE sensor exhibited good selectivity, reproducibility, and stability. It successfully detected RF, DA, and Trp in serum and milk samples with satisfactory results.