Journal
MOLECULAR & CELLULAR PROTEOMICS
Volume 15, Issue 10, Pages 3190-3202Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.M116.060145
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Funding
- National Program on Key Basic Research Project [2014CBA02001, 2012CB910300]
- Program of International ST Cooperation [2012DFB30080, 2014DFB30010, 2014DFB30020]
- National High-tech R&D Program of China [2012AA020201]
- National Natural Science Foundation of China [31200582, 31170779, 31200992, 31270822]
- Beijing Natural Science Foundation [5132012, Z131100005213003]
- National Key Research & Development Plan [2016YFA0502500, 2016YFC0901905]
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Parenchymatous organs consist of multiple cell types, primarily defined as parenchymal cells (PCs) and non-parenchymal cells (NPCs). The cellular characteristics of these organs are not well understood. Proteomic studies facilitate the resolution of the molecular details of different cell types in organs. These studies have significantly extended our knowledge about organogenesis and organ cellular composition. Here, we present an atlas of the cell-type-resolved liver proteome. In-depth proteomics identified 6000 to 8000 gene products (GPs) for each cell type and a total of 10,075 GPs for four cell types. This data set revealed features of the cellular composition of the liver: (1) hepatocytes (PCs) express the least GPs, have a unique but highly homogenous proteome pattern, and execute fundamental liver functions; (2) the division of labor among PCs and NPCs follows a model in which PCs make the main components of pathways, but NPCs trigger the pathways; and (3) crosstalk among NPCs and PCs maintains the PC phenotype. This study presents the liver proteome at cell resolution, serving as a research model for dissecting the cell type constitution and organ features at the molecular level.
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