Journal
MODERN RHEUMATOLOGY
Volume 26, Issue 6, Pages 923-928Publisher
SPRINGER
DOI: 10.3109/14397595.2016.1159385
Keywords
CDR3; Common antigen; IgG4-related disease
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Funding
- Research Program of Intractable Diseases of the Ministry of Health, Labor, and Welfare of Japan
- JSPS KAKENHI Grant [26461487]
- Grants-in-Aid for Scientific Research [26461487] Funding Source: KAKEN
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Objective: We investigated class switch reaction (CSR) in affected organs and evaluated whether the same or genetically related clones exist in IgG4-RD. Methods: We studied three patients with IgG4-RD. Total cellular RNA was extracted from salivary glands and peripheral blood and lung tissue. Activation-induced cytidine deaminase (AID) and immunoglobulin heavy chain third complementarity determining region (IgVH-CDR3) of IgM and IgG4 were detected by reverse transcription polymerase chain reaction (RT-PCR). We analyzed the clonal relationship of infiltrating IgG4-positive cells, as compared with IgM. We determined the existence of common clones among organs and patients. Result: AID was expressed in salivary glands of all patients and lung tissue in one. Closely related IgVH-CDR3 sequences in infiltrating IgG4-positive cells were detected in salivary glands and lung tissue. Identical IgVH-CDR3 sequence between IgM and IgG4 in salivary glands was detected in one patient, indicating CSR in salivary glands. Identical IgVH-CDR3 sequences of IgG4-positive cells were detected between salivary glands and peripheral blood in two patients. Four identical sequences of IgVH-CDR3 existed between patients. Interestingly, one of the four sequences was detected in all patients. Conclusion: Our results demonstrate the existence of common antigen(s) shared by patients with IgG4-RD.
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