Studying Hair Growth Cycle and its Effects on Mouse Skin

Researchers should be aware that hair growth cycle drives prominent molecular, cellular, and morphological changes to the entire skin. Thus, hair growth constitutes a major experimental variable that influences the interpretation of dermatological studies. Hair growth in mice is neither asynchronous nor fully synchronized; rather, it occurs in waves that dynamically propagate across the skin. In consequence, any given area of mouse skin can contain hair follicles in different stages of the cycle in close physical proximity. Furthermore, hair growth waves in mice are initiated by probabilistic events at different time points and across stochastic locations. The consequence of such stochasticity is that precise patterns of hair growth waves differ from mouse to mouse, even in littermates of the same sex. However, such physiological stochasticity is commonly misconstrued as a significant hair growth phenotype in mutant mice or in drug-treated mice. The purpose of this article is to provide a set of guidelines for designing reliably interpretable murine studies on hair growth and to highlight key experimental caveats to be avoided. It also informs on how to account for and minimize the impact of physiological hair cycle differences when designing and interpreting nonhair growth dermatological studies in mice.

Automated summary

Researchers should take into account the impact of the hair growth cycle on the interpretation of dermatological studies. Hair growth in mice is not synchronized and occurs in waves, resulting in different stages of hair follicles being present in close proximity. The stochasticity of hair growth waves in mice can lead to misconceptions in mutant or drug-treated mice. This article provides guidelines for designing reliable murine hair growth studies and highlights experimental caveats to be avoided.