Hemocyte-like cells in larvae of the freshwater snail Biomphalaria glabrata (Mollusca: Panpulmonata)

Despite undergoing development within a germfree egg capsule, embryos and larvae of the freshwater snail Biomphalaria glabrata possess passive immune protection in the form of parentally-derived antimicrobial proteins in the perivitelline fluid. However, the point at which larvae begin to form their own internal defense system (IDS), which consists of both plasma proteins and hemocytes, is not known. In this study, hemocyte-like cells were observed in mechanically-disrupted late trochophores and veligers of the BS-90 strain of B. glabrata. These cells showed the properties of glass adherence, spreading, motility, and binding and phagocytosing polystyrene microspheres. No hemocyte-like cells were recovered from the early trochophore stage, and therefore their formation first occurs during subsequent maturation. Numbers of hemocyte-like cells increased during larval development. Although the functional significance of these cells is not known, they may represent the initial cellular component of the IDS.

Automated summary

During the development of Biomphalaria glabrata, embryos and larvae possess passive immune protection through parentally-derived antimicrobial proteins. This study observed hemocyte-like cells in late trochophores and veligers of the BS-90 strain, suggesting the formation of the internal defense system (IDS). These cells showed various cellular functions and increased in number during larval development.