The SlWRKY57-SlVQ21/SlVQ16 module regulates salt stress in tomato

Salt stress is a major abiotic stress which severely hinders crop production. However, the regulatory network controlling tomato resistance to salt remains unclear. Here, we found that the tomato WRKY transcription factor WRKY57 acted as a negative regulator in salt stress response by directly attenuating the transcription of salt-responsive genes (SlRD29B and SlDREB2) and an ion homeostasis gene (SlSOS1). We further identified two VQ-motif containing proteins SlVQ16 and SlVQ21 as SlWRKY57-interacting proteins. SlVQ16 positively, while SlVQ21 negatively modulated tomato resistance to salt stress. SlVQ16 and SlVQ21 competitively interacted with SlWRKY57 and antagonistically regulated the transcriptional repression activity of SlWRKY57. Additionally, the SlWRKY57-SlVQ21/SlVQ16 module was involved in the pathway of phytohormone jasmonates (JAs) by interacting with JA repressors JA-ZIM domain (JAZ) proteins. These results provide new insights into how the SlWRKY57-SlVQ21/SlVQ16 module finely tunes tomato salt tolerance.

Automated summary

This study reveals that the tomato WRKY transcription factor WRKY57 negatively regulates salt stress response by attenuating the transcription of salt-responsive genes and an ion homeostasis gene. The VQ-motif containing proteins, SlVQ16 and SlVQ21, interact with WRKY57 and modulate tomato's resistance to salt stress, with SlVQ16 acting positively and SlVQ21 acting negatively. The SlWRKY57-SlVQ21/SlVQ16 module is also involved in the jasmonate pathway.