Journal
JOURNAL OF INFECTIOUS DISEASES
Volume -, Issue -, Pages -Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiad221
Keywords
bacteria; diagnostic; respiratory illness; sputum; viruses
Categories
Ask authors/readers for more resources
The BioFire FilmArray Pneumonia Panel (BioFire PN) is a useful tool for diagnosing lower respiratory tract infections by accurately identifying bacterial and viral pathogens, as well as atypical bacteria.
Background Inability to identify the microbial etiology of lower respiratory tract infection leads to unnecessary antibiotic use. We evaluated the utility of the BioFire FilmArray Pneumonia Panel (BioFire PN) to inform microbiologic diagnosis. Methods Hospitalized adults with respiratory illness were recruited; sputa and clinical/laboratory data were collected. Sputa were cultured for bacteria and tested with BioFire PN. Microbial etiology was adjudicated by 4 physicians. Bacterial polymerase chain reaction (PCR) was compared with culture and clinical adjudication. Results Of 298 sputa tested, BioFire PN detected significantly more pathogens (350 bacteria, 16 atypicals, and 164 viruses) than sputum culture plus any standard-of-care testing (91% vs 60%, P < .0001). When compared with culture, the sensitivity of BioFire PN for individual bacteria was 46% to 100%; specificity, 61% to 100%; and negative predictive value, 92% to 100%. Cases were adjudicated as viral (n = 58) and bacterial (n = 100). PCR detected bacteria in 55% of viral cases and 95% of bacterial (P < .0001). High serum procalcitonin and bacterial adjudication were more often associated with sputa with 10(6) or 10(7) copies detected. Conclusions Multiplex PCR testing of sputa for bacteria is useful to rule out bacterial infection with added value to detect viruses and atypical bacteria. BioFire FilmArray Pneumonia Panel sputa testing is useful to rule out bacterial infection with increased yield of viruses and atypical bacteria. Combined with bacterial copy number and clinical variables, the assay could improve antibiotic management of respiratory infections.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available