4.7 Review

Latest Advances in the Application of Humanized Mouse Model for Staphylococcus aureus

Related references

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Article Biotechnology & Applied Microbiology

PD-1/PD-L1 blockade is a potent adjuvant in treatment of Staphylococcus aureus osteomyelitis in mice

Kaiqun Li et al.

Summary: This study found that there is no effective therapy for implant-associated Staphylococcus aureus osteomyelitis, a devastating complication after orthopedic surgery. Immune-suppressive molecules PD-1/PD-L1 were up-regulated in macrophages surrounding the abscess in S. aureus-infected bone, leading to activation of mitophagy and suppression of bactericidal function. Blocking or knockout of PD-1/PD-L1 significantly reduced mitophagy, enhanced bacterial clearance, and reduced bone destruction.

MOLECULAR THERAPY (2023)

Article Immunology

Next-generation humanized NSG-SGM3 mice are highly susceptible to Staphylococcus aureus infection

Sophia Hung et al.

Summary: Humanized mice have shown increased vulnerability to Staphylococcus aureus infection compared to wild type mice, and this study found that next-generation humanized mice with improved myeloid reconstitution were even more susceptible. The severity of infection was correlated with the rate of humanization. These findings have implications for therapy approaches and analysis of virulence mechanisms.

FRONTIERS IN IMMUNOLOGY (2023)

Review Biotechnology & Applied Microbiology

The crafty opponent: the defense systems of Staphylococcus aureus and response measures

Hongjie Hou et al.

Summary: Understanding the defensive systems of Staphylococcus aureus can aid in the optimal use of antimicrobial drugs and guide antimicrobial stewardship. In addition to developing new medicines, alternative techniques like anti-virulence and bacteriophage therapies are being explored as potential tools to combat staphylococcal infections.

FOLIA MICROBIOLOGICA (2022)

Article Biochemistry & Molecular Biology

VraSR Regulatory System Contributes to the Virulence of Community-Associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA) in a 3D-Skin Model and Skin Infection of Humanized Mouse Model

Nilakshi Barua et al.

Summary: The inactivation of the VraSR system in Staphylococcus aureus reduces virulence and stress response. In a 3D-skin model, the inactivated VraSR strains showed decreased adhesion, internalization, and apoptosis. In a humanized mouse infection model, the inactivation of VraSR resulted in smaller skin lesions and reduced bacterial burden.

BIOMEDICINES (2022)

Article Cell Biology

Merlin functions as a critical regulator in Staphylococcus aureus-induced osteomyelitis

Zubin Zhou et al.

Summary: Merlin deficiency alleviates S. aureus-induced osteomyelitis through the regulation of DCAF1, resulting in the suppression of inflammatory responses in macrophages and BMDMs. This indicates a potential therapeutic target for osteomyelitis.

JOURNAL OF CELLULAR PHYSIOLOGY (2022)

Article Chemistry, Medicinal

miR-146a Protects against Staphylococcus aureus-Induced Osteomyelitis by Regulating Inflammation and Osteogenesis

Chaolai Jiang et al.

Summary: miR-146a protects against S. aureus-induced osteomyelitis by regulating inflammation and osteogenesis.

ACS INFECTIOUS DISEASES (2022)

Article Microbiology

Non-protective immune imprint underlies failure of Staphylococcus aureus IsdB vaccine

Chih-Ming Tsai et al.

Summary: Natural exposure to Staphylococcus aureus (SA) may explain the failure of anti-SA immunization trials. Animals infected with SA do not mount protective antibody responses to vaccination, unlike naive animals.

CELL HOST & MICROBE (2022)

Article Immunology

MRSA Infection in the Thigh Muscle Leads to Systemic Disease, Strong Inflammation, and Loss of Human Monocytes in Humanized Mice

Sophia Hung et al.

Summary: MRSA is the second-leading cause of deaths by antibiotic-resistant bacteria globally, and there have been over 100,000 deaths annually. Despite the urgent need to develop a vaccine to control this pathogen, all clinical trials with effective pre-clinical candidates have failed so far. The recent development of humanized mice could help to bridge the gap between pre-clinical evaluation and the clinical situation.

FRONTIERS IN IMMUNOLOGY (2022)

Article Immunology

IL-17 and IL-22 are pivotal cytokines to delay wound healing of S. aureus and P. aeruginosa infected skin

Jean-Claude Lecron et al.

Summary: This study found that the proinflammatory cytokines IL-22 and IL-17 induced by bacterial infection delay the healing process of skin wounds, regardless of the presence of bacteria. This discovery reveals the underlying mechanisms of bacterial infection on wound healing.

FRONTIERS IN IMMUNOLOGY (2022)

Article Orthopedics

Diflunisal-loaded poly(propylene sulfide) nanoparticles decrease S. aureus-mediated bone destruction during osteomyelitis

Caleb A. Ford et al.

Summary: The study demonstrates that diflunisal-loaded PPS nanoparticles effectively reduce Staphylococcus aureus-mediated bone destruction, suggesting the feasibility of systemic delivery of antivirulence compounds to mitigate bone pathology during osteomyelitis.

JOURNAL OF ORTHOPAEDIC RESEARCH (2021)

Article Microbiology

Interaction between Staphylococcus Agr virulence and neutrophils regulates pathogen expansion in the skin

Masanori Matsumoto et al.

Summary: The growth and invasion of Staphylococcus aureus in the skin are differently regulated by Agr and PSM alpha, with Agr limiting intracellular killing within neutrophils to promote pathogen expansion in the dermis and subcutaneous tissue.

CELL HOST & MICROBE (2021)

Article Biotechnology & Applied Microbiology

Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis

Fan Zhang et al.

Summary: Cell-free supernatant of Bacillus subtilis (B. subtilis CFS) can effectively kill Staphylococcus aureus, increase its susceptibility to penicillin and gentamicin, suppress gene expression, alter membrane components, and enhance membrane permeabilization. This suggests that B. subtilis CFS may be a potential resistance-modifying agent against beta-lactam antibiotics for S. aureus.

MICROBIAL CELL FACTORIES (2021)

Review Infectious Diseases

Staphylococcus aureus clones causing osteomyelitis: a literature review (2000-2020)

Fernanda Pimentel de Araujo et al.

Summary: This study aimed to address the global epidemiology of S. aureus clones from osteomyelitis (OM) patients, finding pandemic clones such as CC5, CC8, CC22, CC30, and CC45 were the most common in OM, with significant differences in clone distribution among different regions, and PVL-positive MRSA clones were most common in pediatric patients in Europe and the USA.

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE (2021)

Article Immunology

Humanized Mice Exhibit Exacerbated Abscess Formation and Osteolysis During the Establishment of Implant-Associated Staphylococcus aureus Osteomyelitis

Gowrishankar Muthukrishnan et al.

Summary: The study found that engrafting human hematopoietic stem cells into NSG mice better mimicked human S. aureus bone infections. Research on infected mice showed increased numbers of human T cells during infection, clustering around Staphylococcal abscess communities, indicating activation and proliferation by S. aureus.

FRONTIERS IN IMMUNOLOGY (2021)

Article Cell Biology

Staphylococcus aureus uses the ArlRS and MgrA cascade to regulate immune evasion during skin infection

Jakub M. Kwiecinski et al.

Summary: The immune evasion of Staphylococcus aureus in the skin is controlled by the ArlRS two-component regulatory system and its downstream effector MgrA. Mutants lacking either ArlRS or MgrA are less virulent, unable to form correct abscess structure, and have decreased expression of immune evasion factors, making them susceptible to host defenses. ArlRS and MgrA are crucial regulators of S. aureus immune evasion and potential treatment targets.

CELL REPORTS (2021)

Article Medicine, General & Internal

Adenosine synthase A contributes to recurrent Staphylococcus aureus infection by dampening protective immunity

Jian Deng et al.

Summary: The study demonstrates that the Staphylococcus aureus virulence factor AdsA alters Th17 responses by interfering with NLRP3 inflammasome-mediated IL-1 beta production, dampening Th1/Th17 immunity and promoting recurrent S. aureus infections. These findings provide important insights for therapeutic and vaccination strategies against S. aureus infections.

EBIOMEDICINE (2021)

Article Biochemistry & Molecular Biology

Generation and expression analysis of BAC humanized mice carrying HLA-DP401 haplotype

Feng Li et al.

Summary: This study utilized BAC transgenic humanized mice and developed a model of Staphylococcus aureus pneumonia to analyze the expression of HLA-DPA1/DPB1 genes. The results demonstrated the potential of these transgenic humanized mice in developing animal models for infectious diseases and MHC-associated immunological diseases.

ANIMAL MODELS AND EXPERIMENTAL MEDICINE (2021)

Review Immunology

Pathogenicity and virulence of Staphylococcus aureus

Gordon Y. C. Cheung et al.

Summary: Staphylococcus aureus is a common pathogen causing a range of diseases, with treatment complicated by antibiotic resistance and lack of an effective vaccine. Understanding the role of S. aureus virulence factors can help in developing anti-virulence strategies to combat the challenges posed by this pathogen.

VIRULENCE (2021)

Article Microbiology

A mouse model of Staphylococcus aureus small intestinal infection

Sarah Larcombe et al.

JOURNAL OF MEDICAL MICROBIOLOGY (2020)

Article Multidisciplinary Sciences

Exploiting species specificity to understand the tropism of a human-specific toxin

K. M. Boguslawski et al.

SCIENCE ADVANCES (2020)

Review Medicine, Research & Experimental

Innovations, challenges, and minimal information for standardization of humanized mice

Renata Stripecke et al.

EMBO MOLECULAR MEDICINE (2020)

Review Biochemistry & Molecular Biology

Of men in mice: the development and application of a humanized gnotobiotic mouse model for microbiome therapeutics

John Chulhoon Park et al.

EXPERIMENTAL AND MOLECULAR MEDICINE (2020)

Article Multidisciplinary Sciences

Development of humanized mouse and rat models with full-thickness human skin and autologous immune cells

Yash Agarwal et al.

SCIENTIFIC REPORTS (2020)

Article Cardiac & Cardiovascular Systems

Staphylococcus aureus endocarditis: distinct mechanisms of bacterial adhesion to damaged and inflamed heart valves

Laurens Liesenborghs et al.

EUROPEAN HEART JOURNAL (2019)

Article Medicine, Research & Experimental

Staphylococcus aureus drives expansion of low-density neutrophils in diabetic mice

Taylor S. Cohen et al.

JOURNAL OF CLINICAL INVESTIGATION (2019)

Article Chemistry, Medicinal

Multimodal Tracking of Controlled Staphylococcus aureus Infections in Mice

Mick M. Welling et al.

ACS INFECTIOUS DISEASES (2019)

Article Immunology

Humanized immune system mouse models: progress, challenges and opportunities

Todd M. Allen et al.

NATURE IMMUNOLOGY (2019)

Review Cell Biology

Humanized mice: A brief overview on their diverse applications in biomedical research

Shigeyoshi Fujiwara

JOURNAL OF CELLULAR PHYSIOLOGY (2018)

Review Immunology

Humanized Mice as Unique Tools for Human-Specific Studies

Kylie Su Mei Yong et al.

ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS (2018)

Review Infectious Diseases

Essentials in the management of S-aureus bloodstream infection

N. Jung et al.

INFECTION (2018)

Article Veterinary Sciences

Activation of mast cells in skin abscess induced by Staphylococcus aureus (S. aureus) infection in mice

Zhiqiang Lei et al.

RESEARCH IN VETERINARY SCIENCE (2018)

Article Medicine, General & Internal

Methicillin-resistant Staphylococcus aureus

Andie S. Lee et al.

NATURE REVIEWS DISEASE PRIMERS (2018)

Review Microbiology

Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology

Sahreena Lakhundi et al.

CLINICAL MICROBIOLOGY REVIEWS (2018)

Review Microbiology

Leukocidins: staphylococcal bi-component pore-forming toxins find their receptors

Andras N. Spaan et al.

NATURE REVIEWS MICROBIOLOGY (2017)

Review Immunology

Humanized Mouse Models of Staphylococcus aureus infection

Dane Parker

FRONTIERS IN IMMUNOLOGY (2017)

Review Microbiology

Staphylococcus aureus Infections: Epidemiology, Pathophysiology, Clinical Manifestations, and Management

Steven Y. C. Tong et al.

CLINICAL MICROBIOLOGY REVIEWS (2015)

Review Infectious Diseases

Host factors that contribute to recurrent staphylococcal skin infection

Christopher P. Montgomery et al.

CURRENT OPINION IN INFECTIOUS DISEASES (2015)

Review Microbiology

Models matter: the search for an effective Staphylococcus aureus vaccine

Wilmara Salgado-Pabon et al.

NATURE REVIEWS MICROBIOLOGY (2014)

Article Multidisciplinary Sciences

Characterization of a Mouse-Adapted Staphylococcus aureus Strain

Silva Holtfreter et al.

PLOS ONE (2013)

Article Multidisciplinary Sciences

Genomic responses in mouse models poorly mimic human inflammatory diseases

Junhee Seok et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Article Biotechnology & Applied Microbiology

Will there ever be a universal Staphylococcus aureus vaccine?

Gerald B. Pier

HUMAN VACCINES & IMMUNOTHERAPEUTICS (2013)

Review Immunology

Immunopathogenesis of Staphylococcus aureus pulmonary infection

Dane Parker et al.

SEMINARS IN IMMUNOPATHOLOGY (2012)

Editorial Material Immunology

Inferring reasons for the failure of Staphylococcus aureus vaccines in clinical trials

Fabio Bagnoli et al.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2012)

Article Multidisciplinary Sciences

Human IL-3/GM-CSF knock-in mice support human alveolar macrophage development and human immune responses in the lung

Tim Willinger et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)

Article Immunology

A Postinfluenza Model of Staphylococcus aureus Pneumonia

Mei-Ho Lee et al.

JOURNAL OF INFECTIOUS DISEASES (2010)

Review Food Science & Technology

Exfoliative Toxins of Staphylococcus aureus

Michal Bukowski et al.

TOXINS (2010)

Review Immunology

Of mice and not men: Differences between mouse and human immunology

J Mestas et al.

JOURNAL OF IMMUNOLOGY (2004)

Article Medicine, General & Internal

Nasal carriage as a source of Staphylococcus aureus bacteremia.

C von Eiff et al.

NEW ENGLAND JOURNAL OF MEDICINE (2001)