Journal
MITOCHONDRION
Volume 27, Issue -, Pages 40-47Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2016.02.004
Keywords
mtDNA; D-loop; Methylation; Bisulfite sequencing; Endothelial Cell; Senescence
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Funding
- Fondazione Monzino
- Centro Cardiologico Monzino-IRCCS [RC2012-2015]
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The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5mC clusters and a biased distribution of 5mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5mC in D-loop formation, rather than in mitochondrial gene expression. (C) 2016 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
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