Polystyrene nanoplastics induce developmental impairments and vasotoxicity in zebrafish (Danio rerio)

The exponential use of plastics has significantly increased environmental pollution by nanoplastics (NPs). In the aquatic environment, NPs interact and bioaccumulate in the biota, posing a potential ecotoxicological risk. The present study investigated the developmental toxicity, vasotoxicity, cytotoxicity, ROS induction, and behavioral impairments in zebrafish (Danio rerio) exposed to environmentally relevant polystyrene NPs (PS-NPs) concen-trations (0.04, 34 ng L-1, and 34 mu g L-1) for 144 h through multiple biomarkers response (mortality, frequency of spontaneous contractions, heart rate, and morphological changes). Furthermore, vasotoxicity (head, yolk sac, tail, and branchial vessels) was evaluated using the transgenic zebrafish tg(Fli1:eGFP). Results showed that PS-NPs interacted mainly with zebrafish chorion, gills, tail, and larvae head. PS-NPs at 34 ng L-1 and 34 mu g L-1 induced neurotoxicity (decreased frequency of spontaneous contractions), cardiotoxicity (bradycardia), and morphological changes in the eyes and head, indicating that PS-NPs induce developmental impairments in zebrafish. In addition, cytotoxicity in the caudal region (34 ng L-1), ROS production, decreased mean swimming speed, and distance covered were observed in all tested concentrations. PS-NPs also induced vasotoxicity (yolk sac region) in transgenic zebrafish. Overall, the present study demonstrates the harmful effects of PS-NPs on the early developmental stages of freshwater fish, indicating their environmental risk.

Automated summary

The exponential increase in the use of plastics has led to significant environmental pollution caused by nanoplastics. This study investigated the harmful effects of environmentally relevant polystyrene nanoparticles on the early developmental stages of zebrafish. The results showed that the nanoparticles induced developmental impairments, neurotoxicity, cardiotoxicity, cytotoxicity, and behavioral impairments in the fish. Overall, the findings highlight the environmental risks associated with polystyrene nanoparticles.