Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 457, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2023.131823
Keywords
Pretreatment; Magnetic separation; Pathogenic bacteria; MXene@Au; Biosensor
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This study introduces a magnetic separation-based electrochemical biosensor for the detection of Methicillin-resistant Staphylococcus aureus (MRSA). The sensor showed good stability and applicability, with a detection limit of 3.8 x 101 CFU/mL. MRSA was successfully detected in cerebrospinal fluid samples with satisfactory recoveries and validation results. Overall, this study presents a promising method for the detection of MRSA, with the potential to be further developed into a universal pathogen detection method.
Pathogenic bacteria are associated with high morbidity rates and present significant diagnostic challenges in terms of rapid detection. This study introduces a magnetic separation-based electrochemical biosensor for the detection of Methicillin-resistant Staphylococcus aureus (MRSA). Vancomycin (Van) was used to modify on the surface of polyethyleneimine (PEI) mediated MBs (MBs-PEI-Van) for separation and enrichment of MRSA. The MBs-PEI-Van shown a satisfactory stability and applicability with capture effective (CE) > 85% in both PBS and cerebrospinal fluid (CSF) samples. MXene@Au with controllable size of AuNPs was synthesized by a selfreduction method and employed to modify the glassy carbon electrode (GCE). Immunoglobulin G (IgG) was loaded onto the modified electrode to immobilize MRSA, and ferroceneboronic acid (Fc-BA) was used as a probe for quantitative determination. The differential pulse voltammetry (DPV) current was plotted against the concentration of MRSA from 3.8 x 101 to 3.8 x 107 CFU/mL with a limit of detection (LOD) of 3.8 x 101 CFU/mL. In addition, MRSA was successfully detected in spiked CSF samples with satisfactory recoveries (94.35-107.81 %) and validation results (RSD < 11 %). Overall, this study presents a promising method for the detection of MRSA, with the potential to be further developed into a universal pathogen detection method.
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