Polydiacetylene based colorimetric nano-sensor for on-spot detection of aflatoxin in food matrices and animal feed

Global food security is constantly being challenged by the prevalence of aflatoxin (AF), a carcinogenic mycotoxin found in various staple foods consumed across the globe. This challenge is further compounded by unavailability of convenient, on-field aflatoxin sensing devices. In this study, liposome sensors conjugated with polydiacetylene (PDA) have been developed for robust, low-cost, on-site quantification of aflatoxin from feed samples assisted by a convenient mobile phone-based application. Soyabean-sourced phosphatidylcholine (content & GE; 94.0%) demonstrated to be a stronger structural backbone for developed lipoid sensor and is comparatively cheaper and sustainable alternative to conventional backbones like synthetic 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and dipalmitoylphosphatidylcholine (DPPC) for the preparation of based liposomes. Anti-aflatoxin antibodies conjugated with chemically activated liposomes provide a consistent chromatic response with aflatoxin. These sensors were used to detect the presence of aflatoxin in food and animal feed matrices. The detection time of the developed sensor was 5 min and can provide rugged and reliable detection of AFB1 up to the limit of 5.7 parts per billion (ppb).

Automated summary

Liposome sensors conjugated with polydiacetylene (PDA) have been developed for on-site quantification of aflatoxin from feed samples with the assistance of a mobile phone-based application. The soyabean-sourced phosphatidylcholine served as a stronger and cheaper alternative backbone for the lipoid sensor, providing consistent chromatic response with aflatoxin. These sensors can detect the presence of aflatoxin in food and animal feed matrices within 5 minutes, with a reliable detection limit of 5.7 ppb.