4.6 Article

Profiling of regioisomeric triacylglycerols in pistachio nuts by high-performance liquid chromatography-electrospray ionization mass spectrometry

Journal

JOURNAL OF FOOD COMPOSITION AND ANALYSIS
Volume 122, Issue -, Pages -

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2023.105395

Keywords

Pistachio; (Pistacia vera L; ); Triacylglycerols (TAGs); Lipids; Liquid chromatography (LC); Tandem mass spectroscopy; Retention time m; z map

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A non-aqueous reversed-phase liquid chromatographic method coupled to electrospray ionization tandem mass spectrometry was used to identify triacylglycerols (TAGs) in pistachio lipids at the regioisomeric level. A two-dimensional (2D) map was employed for effective profiling of TAGs, specifically odd-numbered fatty acids and long-chain derivatives. TAGs were observed as pairs of regioisomers with distinct retention times and tandem mass spectra. Over 100 TAGs containing 26 fatty acids with 51-63 carbon atoms and 0-3 double bonds were identified in different pistachio samples. This method provides a fast and selective approach for quantifying complex TAGs in pistachio samples.
In this contribution, a non-aqueous reversed-phase liquid chromatographic method coupled to electrospray ionization (ESI) tandem mass spectrometry (LC-MS) is used for the identification of triacylglycerol (TAG) down to the regioisomersic level in pistachio lipids. Furthermore, for effective profiling of TAG, a two-dimensional (2D) map was employed to identify the entire homologous series of TAGs especially, odd-numbered fatty acid and long-chain derivatives. Notably, TAGs were observed as pairs of regioisomers with distinct retention times and tandem mass spectra. Furthermore, more than 100 TAGs containing 26 fatty acids with 51-63 carbon atoms and 0-3 double bonds were identified in different pistachio samples. The developed method is effective and facile for the fast quantification of complex TAGs in pistachio samples at high selectivity.

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