4.7 Article

A ratiometric fluorescent system for carboxylesterase detection with AIE dots as FRET donors

Journal

CHEMICAL COMMUNICATIONS
Volume 51, Issue 64, Pages 12791-12794

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc04771d

Keywords

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Funding

  1. National Key Basic Research Program of China [2013CB834702]
  2. NSFC [21474031, 21174040, 21025415]
  3. Science and Technology Planning Project of Guangdong Province [2014A010105009]

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A ratiometric fluorescent system for CaE detection with AIE dots as the FRET donors was designed. Upon enzymatic reaction, electrostatic interaction between the cationic TPE-N+ dots and the enzymatic reaction product - the negatively charged fluorescein molecules allows the FRET process to proceed, thus affording the ratiometric fluorescence CaE assay.

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