Cutaneous cryptococcosis due to Cryptococcus neoformans with the formation of a giant subcutaneous nodule

Summary: The first case of cutaneous Papiliotrema (Cryptococcus) laurentii infection in a 23-year-old Caucasian woman with autoimmune thyroiditis and hypothyroidism is reported. Immune system impairments are often associated with unusual fungal infections.

Summary: This study re-evaluated sample pre-processing procedures for yeasts of the Cryptococcus neoformans/gattii species complexes and created a set of publicly available references for use with the MALDI Biotyper system. By assessing peak content using four different pre-processing protocols and creating 13 reference entries with standard formic acid extraction, correct classification of 93.5% of test isolates was achieved. The freely available database references can improve species-level identification of cryptococcal isolates.