Application of cellulose to chromatographic media: Cellulose dissolution, and media fabrication and derivatization

As the cornerstone of chromatographic technology, the development of high-performance chromatographic media is a crucial means to enhance the purification efficiency of biological macromolecules. Cellulose is a popular biological separation medium due to its abundant hydroxyl group on the surface, easy modification and, weak non-specific adsorption. In this paper, the development of cellulosic solvent systems, typical preparation methods of cellulosic chromatographic media, and the enhancement of chromatographic properties of cellulosic chromatographic media by polymeric ligand grafting strategies and their mechanism of action are reviewed. Ultimately, based on the current research status, a promising outlook for the preparation of high-performance cellulose-based chromatographic media was presented. & COPY; 2023 Elsevier B.V. All rights reserved.

Automated summary

The development of high-performance chromatographic media is essential in improving the efficiency of purifying biological macromolecules. Cellulose is a popular choice due to its abundant hydroxyl group, easy modification, and weak non-specific adsorption. This paper reviews the development of cellulose solvent systems, typical preparation methods of cellulose chromatographic media, and the enhancement of their properties through polymeric ligand grafting strategies.