Journal
MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS
Volume 23, Issue 5, Pages 1237-1245Publisher
SPRINGER
DOI: 10.1007/s00542-016-2874-7
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Funding
- Malaysian Ministry of Higher Education (MOHE) through the FRGS Grant [9003-00276]
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Non toxicity and high isoelectric point of zinc oxide nanorods (ZnO NRs) are the promising materials widely used in biomolecule detection and biomedicine application. An electrochemical DNA biosensor was successfully fabricated by depositing (3-Aminopropyl)triethoxysilane and gold nanoparticles (GNPs) on the hydrothermal growth ZnO NRs. The modified surface with GNPs (35 +/- 5 nm) was characterized by field emission scanning electron microscopy with energy dispersive X-ray and X-ray diffraction. The fabricated electrode was used for the label-free detection of DNA. Thiolated (-SH) single stranded DNA was immobilized onto the GNPs surface to form a gold-thiolate bond. Hybridization with complementary target DNA was performed with the presence of ruthenium complex. Immobilization and hybridization detection were performed using A mu AUTOLAB with cyclic voltammetric software. The measured sensitivity of the fabricated electrode for hybridization is up to 1.6 times larger than immobilization.
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