Rapid On-Site Detection Techniques and Applications of Norovirus in Paediatric Emergencies

Infants and young children across the globe are most commonly afflicted with acute gastroenteritis (AGE) due to the prevalence of Norovirus, which results in significant morbidity and mortality rate and is highly transmissible. Therefore, a swiftly and accurately detection can aid in minimizing their transmission. The objective of this research was to design a dual LAMP fluorescence detection system for HuNoVs using LAMP technology to detect the ORF1-ORF2 junction, which is the region most conserved in HuNoVs genomes I and II. Using a commercially available integrated detection system based on magnetic bead-based nucleic acid extraction and LAMP fluorescence detection, we built a rapid integrated detection platform for paediatric norovirus emergencies The method was cross reactive with four other viruses. Results from the integrated system agreed with conventional laboratory extracts for 28 clinical samples.

Automated summary

This research aimed to design a dual LAMP fluorescence detection system for HuNoVs, which can accurately detect the ORF1-ORF2 junction region, the most conserved area in HuNoVs genomes I and II. A rapid integrated detection platform for pediatric norovirus emergencies was built using a commercially available system based on magnetic bead-based nucleic acid extraction and LAMP fluorescence detection. The method showed cross-reactivity with four other viruses and demonstrated consistent results with conventional laboratory extracts for 28 clinical samples.