Multiple Metabolism Pathways of Bentazone Potentially Regulated by Metabolic Enzymes in Rice

Bentazone (BNTZ) is a selective and efficient herbicideused incrop production worldwide. However, the persistence of BNTZ residuesin the environment has led to their increasing accumulation in farmlandand crops, posing a high risk to human health. To evaluate its impacton crop growth and environmental safety, a comprehensive study wasconducted on BNTZ toxicity, metabolic mechanism, and resultant pathwaysin rice. The rice growth was compromised to the treatment with BNTZat 0.2-0.8 mg/L (529.95-1060.05 g a.i./ha), while theactivities of enzymes including SOD, POD, CAT, GST, GT, and CYP450were elevated under BNTZ stress. A genome-wide RNA-sequencing (RNA-Seq)was performed to dissect the variation of transcriptomes and metabolicmechanisms in rice exposed to BNTZ. The degradative pathways of BNTZin rice are involved in glycosylation, hydrolysis, acetylation, andconjugation processes catalyzed by the enzymes. Our data providedevidence that helps understand the BNTZ metabolic and detoxic mechanisms.

Automated summary

This study investigated the toxicity, metabolic mechanism, and resultant pathways of bentazone (BNTZ) in rice. It was found that the growth of rice was compromised when treated with BNTZ at 0.2-0.8 mg/L (529.95-1060.05 g a.i./ha), and the activities of certain enzymes were elevated under BNTZ stress. Genome-wide RNA-sequencing analysis revealed that the degradative pathways of BNTZ in rice involve glycosylation, hydrolysis, acetylation, and conjugation processes catalyzed by enzymes. These findings provide valuable insights into the metabolic and detoxic mechanisms of BNTZ.