Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria

Environmental bacteria host an enormous number of prophages, but their diversity and natural functions remain largely elusive. Here, we investigate prophage activity and diversity in 63 Erwinia and Pseudomonas strains isolated from flag leaves of wheat grown in a single field. Introducing and validating Virion Induction Profiling Sequencing (VIP-Seq), we identify and quantify the activity of 120 spontaneously induced prophages, discovering that some phyllosphere bacteria produce more than 108 virions/mL in overnight cultures, with significant induction also observed in planta. Sequence analyses and plaque assays reveal E. aphidicola prophages contribute a majority of intraspecies genetic diversity and divide their bacterial hosts into antagonistic factions engaged in widespread microbial warfare, revealing the importance of prophage-mediated microdiversity. When comparing spontaneously active prophages with predicted prophages we also find insertion sequences are strongly correlated with non-active prophages. In conclusion, we discover widespread and largely unknown prophage diversity and function in phyllosphere bacteria.

Automated summary

This study reveals the widespread and diverse prophages in phyllosphere bacteria and their roles in bacterial interactions. The researchers use a novel sequencing method to identify and quantify spontaneously induced prophages, discovering high levels of prophage activity in both culture and plant environments. They also find that E. aphidicola prophages contribute to intraspecies genetic diversity and divide their bacterial hosts into antagonistic factions engaged in microbial warfare.