Nocardioides potassii sp. nov., isolated from weathered potash tailings soil

A Gram-positive, aerobic actinomycete, designated strain KLBMP 9356T, was isolated from weathered potash tailings soil sampled in Xuzhou, Jiangsu Province, PR China. The colonies were cream-coloured, convex and rounded. The optimal growth conditions of strain KLBMP 9356(T) were 1 % (w/v) NaCl, 28 degrees C and pH 7. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KLBMP 9356(T) showed the highest similarity to Nocardioides zhouii CGMCC 1.11084(T) (98.9 %) and Nocardioides glacieisoli CGMCC 1.11097(T) (98.7 %). Results from two tree-making algorithms supported the position that strain KLBMP 9356(T) forms a stable clade with N. zhouii CGMCC 1.11084(T) and N. glacieisoli CGMCC 1.11097(T). Strain KLBMP 9356T exhibited low digital DNA -DNA hybridization values with N. zhouii CGMCC 1.11084(T) (27.6 %) and N. glacieisoli CGMCC 1.11097(T) (31.4 %). The average nucleotide identity values between strain KLBMP 9356T and N. zhouii CGMCC 1.11084(T) and N. glacieisoli CGMCC 1.11097(T) were 83.8% and 85.9%, respectively. The peptidoglycan in the cell wall of the novel strain was ll-2,6-diaminopimelic acid and the predominant menaquinone was MK-8(H4). The major fatty acids (>10 %) were C17:1?8c and C18:1?9c. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, lyso- phospatidylglycerol and phosphatidylinositol. The genomic DNA G+C content was 71.6 mol%. Based on its morphological, chemotaxonomic and phylogenetic characteristics, strain KLBMP 9356(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides potassii sp. nov. is proposed. The type strain is KLBMP 9356(T) (=CGMCC 4.7738(T)=NBRC 115493(T)).

Automated summary

A Gram-positive, aerobic actinomycete designated strain KLBMP 9356T was isolated from weathered potash tailings soil in Xuzhou, Jiangsu Province, PR China. The strain showed phylogenetic similarity to Nocardioides zhouii CGMCC 1.11084T (98.9%) and Nocardioides glacieisoli CGMCC 1.11097T (98.7%). It exhibited distinct characteristics such as cell wall peptidoglycan, menaquinone, fatty acids, and polar lipids. The strain represents a novel species named Nocardioides potassii sp. nov. with a G+C content of 71.6 mol%. Rating: 8/10.