Review of different temperatures for biopreservation

Low-temperature biopreservation is widely used for the storage of medications. As biochemical reactions in cells are slow at low temperatures, they can be stored for longer periods. However, cryopreservation can result in cryo-injury. Therefore, determining how to achieve long-term biopreservation without causing injury has become an essential research topic. Biological tissues should be stored at different temperature zones depending on the purpose of storage and cell characteristics. In order to achieve better storage efficiency, it is critical to investigate and review the best storage temperature for various biomaterials. The mechanisms of cryo-injury and biopreservation are discussed in this review, and the reasons for the preservation of biological tissues at different temperature zones are also reviewed. Based on the review, the storage characteristics of different temperature ranges are summarized, and the future development of biological storage is prospected. This review has some reference significance for biostorage technology.

Automated summary

Low-temperature biopreservation is commonly used for medication storage to prolong their shelf life. However, cryo-injury can occur during cryopreservation, making it crucial to find a way to achieve long-term biopreservation without causing harm. The optimal storage temperature for different biomaterials should be investigated to improve storage efficiency. This review discusses the mechanisms of cryo-injury and biopreservation, as well as the reasons for storing biological tissues at various temperature zones. It provides valuable insights for the development of biostorage technology.