Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/ijms241813955
Keywords
envelope protein; recombinant protein; Zika Virus; rEZIKV; airlift bioreactor; FPLC
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This study successfully obtained a bioactive and functional recombinant envelope protein from Zika Virus (rEZIKV) using an E. coli expression system and an automated fast protein liquid chromatography (FPLC) protocol. The use of an airlift bioreactor for the production of rEZIKV showed great potential for ZIKV vaccine development.
The Zika Virus (ZIKV) is an emerging arbovirus of great public health concern, particularly in the Americas after its last outbreak in 2015. There are still major challenges regarding disease control, and there is no ZIKV vaccine currently approved for human use. Among many different vaccine platforms currently under study, the recombinant envelope protein from Zika Virus (rEZIKV) constitutes an alternative option for vaccine development and has great potential for monitoring ZIKV infection and antibody response. This study describes a method to obtain a bioactive and functional rEZIKV using an E. coli expression system, with the aid of a 5-L airlift bioreactor and following an automated fast protein liquid chromatography (FPLC) protocol, capable of obtaining high yields of approximately 20 mg of recombinant protein per liter of bacterium cultures. The purified rEZIKV presented preserved antigenicity and immunogenicity. Our results show that the use of an airlift bioreactor for the production of rEZIKV is ideal for establishing protocols and further research on ZIKV vaccines bioprocess, representing a promising system for the production of a ZIKV envelope recombinant protein-based vaccine candidate.
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