Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 17, Pages -Publisher
MDPI
DOI: 10.3390/ijms241713233
Keywords
plant mitochondria; pentatricopeptide repeat (PPR) proteins; ATP synthase; RNA processing factor 2 (RPF2); biotechnology
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Researchers created a library of redesigned PPR proteins, related to restorer-of-fertility proteins, and transformed them into plants to target mitochondrial transcripts. Testing 90 different variants in vivo showed a wide range of phenotypes. One specific variant from the library induced specific cleavage of atp1 transcripts, resulting in a phenotype characterized by slow growth and downward curled leaves, validating the use of this library as a source of mitochondrial "mutants". This study is a step towards developing specific RNA targeting tools using PPR proteins.
Pentatricopeptide repeat (PPR) proteins are considered a potential tool for manipulating organelle gene expression in plants because they can recognise a wide range of different RNA sequences, and the molecular basis for this sequence recognition is partially known and understood. A library of redesigned PPR proteins related to restorer-of-fertility proteins was created and transformed into plants in order to target mitochondrial transcripts. Ninety different variants tested in vivo showed a wide range of phenotypes. One of these lines, which displayed slow growth and downward curled leaves, showed a clear reduction in complex V. The phenotype was due to a specific cleavage of atp1 transcripts induced by a modified PPR protein from the library, validating the use of this library as a source of mitochondrial 'mutants'. This study is a step towards developing specific RNA targeting tools using PPR proteins that can be aimed at desired targets.
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