4.7 Article

Surface vs. solution hybridization: effects of salt, temperature, and probe type

Journal

CHEMICAL COMMUNICATIONS
Volume 51, Issue 97, Pages 17245-17248

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc06674c

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Funding

  1. National Institutes of Health [NHGRI R01HG004512]
  2. National Science Foundation [DMR 12-06754]
  3. New York University
  4. Direct For Mathematical & Physical Scien
  5. Division Of Materials Research [1206754] Funding Source: National Science Foundation

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Hybridization thermodynamics on solid supports are compared with those in solution for two types of hybridization probe, DNA and uncharged morpholino oligonucleotides of identical sequences. Trends in hybridization affinity are discussed with respect to ionic strength, temperature, and surface behavior.

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