Photo- and Sono-Active Food Colorants Inactivating Bacteria

Food colorants are commonly used as excipients in pharmaceutical and nutraceutical fields, but they have a wide range of other potential applications, for instance, as cytotoxic drugs or mediators of physical antimicrobial treatments. The photodynamic antibacterial activity of several edible food colorants is reported here, including E127, E129, E124, E122, E133, and E150a, alongside Rhein, a natural lipophilic antibacterial and anticancer compound found in medicinal plants. Minimal inhibitory concentration (MIC) values for S. aureus and E. coli showed that E127 and Rhein were effective against both bacteria, while other colorants exhibited low activity against E. coli. In some cases, dark pre-incubation of the colorants with Gram-positive S. aureus increased their photodynamic activity. Adding Rhein to E127 increased the photodynamic activity of the latter in a supportive mode. Optional sensing mechanism pathways of combined E127/Rhein action were suggested. The antibacterial activity of the studied colorants can be ranged as follows: E127/Rhein >> E127 >> E150a > E122 > E124 >> E129 approximate to E133. E127 was also found to exhibit photodynamic properties. Short ultrasonic treatment before illumination caused intensification of E127 photodynamic activity against E. coli when applied alone and especially in combination with Rhein. Food colorants exhibiting photo- and sonodynamic properties may have good potential in food preservation.

Automated summary

Food colorants have a wide range of potential applications, including as cytotoxic drugs and antimicrobial mediators. This study investigated the photodynamic antibacterial activity of several edible food colorants and Rhein. The results showed that E127 and Rhein were effective against both S. aureus and E. coli, while other colorants exhibited lower activity. Dark pre-incubation with S. aureus increased the photodynamic activity of certain colorants. Adding Rhein to E127 enhanced its photodynamic activity. The study suggests that food colorants with photo- and sonodynamic properties may be useful for food preservation.