Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/ijms241813898
Keywords
papaya; chilling injury; respiration pathway; EMP pathway; TCA cycle
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Storage temperature has an impact on the respiratory metabolism and development of abnormal chilling injury in papaya. Storage at 1 degree Celsius reduces the respiration rate of the EMP-TCA pathway, lowers the consumption of respiratory substrates, and mitigates the occurrence of chilling injury.
Postharvest abnormal chilling injury (CI) behavior in papaya (Carica papaya L.) fruit is a rare phenomenon that may be associated with respiratory metabolism. This study thus aimed to investigate the impacts of storage temperatures (1 and 6 degrees C) on the respiratory metabolism of postharvest papaya and its impact on CI development. Results demonstrated that 1 degrees C storage reduced the activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), citrate synthase (CS), and alpha-ketoglutarate dehydrogenase (alpha-KGDH) and regulated the expression of corre-sponding enzymes in the Embden-Meyerhof-Parnas (EMP) pathway and tricarboxylic acid (TCA) cycle compared with 6 degrees C storage, resulting in a lower respiration rate of the EMP-TCA pathway and mitigating the development of CI. Meanwhile, lower contents of nicotinamide adenine dinucleotide (hydrogen) (NAD(H)) were observed in papaya fruit stored at 1 degrees C. Notably, papaya fruit stored at 1 degrees C maintained higher activity and transcriptional levels of SDH and IDH during the whole storage period. These findings suggest that 1 degrees C storage reduced the respiration rate of the EMP-TCA pathway by reducing the expression level and activity of related enzymes, which is conducive to the reduction of respiration substrate consumption and finally alleviating the occurrence of CI.
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