Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 20, Pages -Publisher
MDPI
DOI: 10.3390/ijms242015453
Keywords
aminoacyl-tRNA synthetase; tryptophan; amino acid transport; mutagenesis; tryptophanyl-tRNA synthetase; tryptophan depletion
Ask authors/readers for more resources
The study shows that Trp-deficient condition is critical for Trp uptake, not only in cells with added TrpRS protein but also in TrpRS-overexpressing cells. Tryptophanyl-AMP production by TrpRS is essential for high-affinity Trp uptake.
Our previous study demonstrated that L-tryptophan (Trp)-depleted cells display a marked enhancement in Trp uptake facilitated by extracellular tryptophanyl-tRNA synthetase (TrpRS). Here, we show that Trp uptake into TrpRS-overexpressing cells is also markedly elevated upon Trp starvation. These findings indicate that a Trp-deficient condition is critical for Trp uptake, not only into cells to which TrpRS protein has been added but also into TrpRS-overexpressing cells. We also show that overexpression of TrpRS mutants, which cannot synthesize tryptophanyl-AMP, does not promote Trp uptake, and that inhibition of tryptophanyl-AMP synthesis suppresses this uptake. Overall, these data suggest that tryptophanyl-AMP production by TrpRS is critical for high-affinity Trp uptake.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available