4.7 Article

mTOR Inhibitors Modulate the Physical Properties of 3D Spheroids Derived from H9c2 Cells

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

G-Protein-Coupled Receptors Mediate Modulations of Cell Viability and Drug Sensitivity by Aberrantly Expressed Recoverin 3 within A549 Cells

Hanae Ichioka et al.

Summary: To investigate the molecular mechanisms responsible for aberrant expression of recoverin (Rec) in cancer cells, Rec-negative lung adenocarcinoma cells (A549) were transfected with a human recoverin cDNA plasmid (A549 Rec) or an empty plasmid (A549 MOCK). The A549 Rec cells showed increased sensitivity to anti-tumor agents, altered cellular metabolism, and formation of larger and stiffer 3D spheroids compared to A549 MOCK. RNA sequencing analysis suggested that aberrantly expressed Rec may be associated with G-protein-coupled receptor (GPCR)-mediated signaling and the cAMP response element binding protein (CREB) signaling pathway.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

Article Biochemistry & Molecular Biology

TGF-β-3 Induces Different Effects from TGF-β-1 and-2 on Cellular Metabolism and the Spatial Properties of the Human Trabecular Meshwork Cells

Megumi Watanabe et al.

Summary: Comparison of the effects of three TGF-beta isoforms on HTM cells revealed that TGF-beta-3 had the most potent effect on TEER and FITC dextran permeability. However, TGF-beta-3 also induced different metabolic effects and had diverse effects on the physical properties and gene expression of ECM components in comparison to TGF-beta-1 and TGF-beta-2. These findings suggest that the unique action of TGF-beta-3 may play a role in the pathogenesis of glaucoma.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

Article Cell Biology

3D Spheroid Configurations Are Possible Indictors for Evaluating the Pathophysiology of Melanoma Cell Lines

Hiroshi Ohguro et al.

Summary: By studying the 3D spheroid structures and cellular metabolism of different MM cell lines, it was found that the degree of deformation varies among different cell lines, and the genes KRAS and SOX2 may play a role in inducing these diverse configurations.

CELLS (2023)

Review Biochemistry & Molecular Biology

Engineered human cardiac tissues for modeling heart diseases

Sungjin Min et al.

Summary: Heart disease is a major global health threat. Three-dimensional cardiac tissue models produced using tissue engineering technology and human cells have shown superior accuracy and reproducibility in disease modeling and drug evaluation, with the potential to recapitulate the structural and functional features of the human heart.

BMB REPORTS (2023)

Article Multidisciplinary Sciences

Enhanced glucose metabolism through activation of HIF-1α covers the energy demand in a rat embryonic heart primordium after heartbeat initiation

Tatsuya Sato et al.

Summary: The initiation of heartbeat in the heart primordium leads to changes in metabolic characteristics, including increased ATP levels and decreased reduced glutathione levels. Glycolysis and ATP synthesis-linked mitochondrial respiration are enhanced, but subunits in complexes of mitochondrial oxidative phosphorylation are not upregulated. HIF-1 alpha is activated and related enzymes in metabolic pathways are upregulated to meet the increased energy demand in the heart primordium.

SCIENTIFIC REPORTS (2022)

Review Medicine, General & Internal

Generating 3D human cardiac constructs from pluripotent stem cells

Chang Liu et al.

Summary: Human pluripotent stem cell (hPSC) technology provides abundant opportunities for disease modeling. Cardiomyocytes derived from hPSCs are efficient tools for studying cardiac diseases, drug screening, and pathological mechanisms. This review discusses the advantages and limitations of the 2D hPSC-cardiomyocyte system and introduces recent developments in 3D culture platforms derived from hPSCs.

EBIOMEDICINE (2022)

Article Cell Biology

STAT3 Is the Master Regulator for the Forming of 3D Spheroids of 3T3-L1 Preadipocytes

Hiroshi Ohguro et al.

Summary: In this study, the differences between 2D and 3D cultured 3T3-L1 preadipocytes were explored using RNA-sequencing analyses. The results showed that 3D spheroids exhibited significant adipogenesis compared to 2D-cultured cells, and the expression levels of adipogenesis-related genes were higher in 3D spheroids. Further analysis revealed that STAT3 was the most critical upstream regulator in regulating the unique biological properties of 3D spheroids.

CELLS (2022)

Article Multidisciplinary Sciences

ROCK 1 and 2 affect the spatial architecture of 3D spheroids derived from human corneal stromal fibroblasts in different manners

Yosuke Ida et al.

Summary: This study examined the roles of ROCK1 and 2 on the spatial architecture of human corneal stroma. The results showed that ROCK2 had a more significant effect than ROCK1 on gene expression and physical properties. Additionally, ROCK1 and 2 had different impacts on the spatial structure of 3D HCSFs spheroids.

SCIENTIFIC REPORTS (2022)

Article Pharmacology & Pharmacy

Nuclear translocation of MLKL enhances necroptosis by a RIP1/RIP3-independent mechanism in H9c2 cardiomyoblasts

Shoya Ino et al.

JOURNAL OF PHARMACOLOGICAL SCIENCES (2022)

Article Biochemistry & Molecular Biology

Human Trabecular Meshwork (HTM) Cells Treated with TGF-β2 or Dexamethasone Respond to Compression Stress in Different Manners

Megumi Watanabe et al.

Summary: Real-time cellular metabolism analysis indicated that TGF-beta 2 significantly induced a shift in cellular energy metabolism, while DEX had a similar but lesser effect. Direct compression stresses on 3D HTM spheroids resulted in fluctuation of gene expressions of ECM proteins, inflammatory cytokines, and ER stress-related factors. However, compression stresses on TGF-beta 2- or DEX-treated 3D HTM spheroids induced downregulation of inflammatory cytokines and ER stress-related factors.

BIOMEDICINES (2022)

Review Biochemistry & Molecular Biology

Overview of Research into mTOR Inhibitors

Beibei Mao et al.

Summary: The mammalian target of rapamycin (mTOR) is a crucial protein kinase involved in various cellular processes. Inhibitors of mTOR have shown antiviral, anti-inflammatory, and antiproliferative effects and have been applied in the research of different diseases.

MOLECULES (2022)

Article Multidisciplinary Sciences

Establishment of appropriate glaucoma models using dexamethasone or TGFβ2 treated three-dimension (3D) cultured human trabecular meshwork (HTM) cells

Megumi Watanabe et al.

Summary: By culturing human trabecular meshwork cells in the presence of dexamethasone or TGF beta 2, researchers were able to establish suitable ex vivo models for studying steroid-induced and primary open-angle glaucoma. These models showed changes in extracellular matrix expression and spheroid size and stiffness, making them valuable tools for glaucoma research.

SCIENTIFIC REPORTS (2021)

Review Biochemistry & Molecular Biology

AMPK-mTOR Signaling and Cellular Adaptations in Hypoxia

Yoomi Chun et al.

Summary: Cellular energy is primarily generated through the oxidative degradation of nutrients and mitochondrial respiration, with oxygen participating in the electron transport chain. Oxygen supply plays a crucial role in intracellular bioenergetics. Hypoxia leads to nutrient starvation, integrating hypoxic signaling and nutrient signaling at the cellular level.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Biochemistry & Molecular Biology

Rosiglitasone and ROCK Inhibitors Modulate Fibrogenetic Changes in TGF-β2 Treated Human Conjunctival Fibroblasts (HconF) in Different Manners

Yuika Oouchi et al.

Summary: The study revealed that TGF beta 2 induced fibrogenetic changes in both 2D and 3D cultured HconF, which were inhibited by Rosi and ROCK inhibitors, respectively.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Review Cell Biology

Remodeling of Cardiac Gap Junctional Cell-Cell Coupling

Stefan Dhein et al.

Summary: The heart relies on gap junction channels to maintain cell-cell coupling for efficient and rapid electrical conduction. Connexin expression and gap junction localization play crucial roles in modulating cardiac electrical properties, ultimately affecting cardiac stability and function.

CELLS (2021)

Article Biochemistry & Molecular Biology

Modulation of the Physical Properties of 3D Spheroids Derived from Human Scleral Stroma Fibroblasts (HSSFs) with Different Axial Lengths Obtained from Surgical Patients

Hiroyasu Katayama et al.

Summary: The study revealed significant differences in the size, stiffness, and expression of extracellular matrix (ECM) molecules in 3D cultures of human scleral stroma fibroblasts (HSSFs) with different axial lengths. In the largest axial length group, the 3D HSSFs spheroids were smaller, less stiff, and showed significant changes in ECM expression compared to other groups. Moreover, the expression of several ECM modulators and endoplasmic reticulum stress-related genes were altered with increasing axial length, providing insights into the molecular mechanisms responsible for myopic changes in the sclera.

CURRENT ISSUES IN MOLECULAR BIOLOGY (2021)

Review Cardiac & Cardiovascular Systems

Targeting Age-Related Pathways in Heart Failure

Li Haobo et al.

CIRCULATION RESEARCH (2020)

Review Medicine, Research & Experimental

Short-chain fatty acid, acylation and cardiovascular diseases

Xiao-Feng Chen et al.

CLINICAL SCIENCE (2020)

Article Ophthalmology

Prostaglandin F2α Agonists Negatively Modulate the Size of 3D Organoids from Primary Human Orbital Fibroblasts2

Kaku Itoh et al.

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2020)

Article Medicine, Research & Experimental

Tanshinone IIA protects against heart failure post-myocardial infarction via AMPKs/mTOR-dependent autophagy pathway

Xuefeng Zhang et al.

BIOMEDICINE & PHARMACOTHERAPY (2019)

Article Multidisciplinary Sciences

HIF-1α is required for development of the sympathetic nervous system

Romana Bohuslavova et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Article Engineering, Biomedical

Cardiovascular disease models: A game changing paradigm in drug discovery and screening

Houman Savoji et al.

BIOMATERIALS (2019)

Article Biochemistry & Molecular Biology

mTORC1 inhibition attenuates necroptosis through RIP1 inhibition mediated TFEB activation

Koki Abe et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2019)

Article Endocrinology & Metabolism

HIF2A-LOX Pathway Promotes Fibrotic Tissue Remodeling in Thyroid-Associated Orbitopathy

Fumihito Hikage et al.

ENDOCRINOLOGY (2019)

Review Cardiac & Cardiovascular Systems

3D Cardiac Cell Culture: A Critical Review of Current Technologies and Applications

Christian Zuppinger

FRONTIERS IN CARDIOVASCULAR MEDICINE (2019)

Editorial Material Cardiac & Cardiovascular Systems

Conceptual Framework for Addressing Residual Atherosclerotic Cardiovascular Disease Risk in the Era of Precision Medicine

Kershaw V. Patel et al.

CIRCULATION (2018)

Review Cardiac & Cardiovascular Systems

New Insights Into the Role of mTOR Signaling in the Cardiovascular System

Sebastiano Sciarretta et al.

CIRCULATION RESEARCH (2018)

Review Cardiac & Cardiovascular Systems

Mitochondrial quality control mechanisms as molecular targets in cardiac ageing

Anna Picca et al.

NATURE REVIEWS CARDIOLOGY (2018)

Review Physiology

Hypoxia-Inducible Factor 1 and Cardiovascular Disease

Gregg L. Semenza

ANNUAL REVIEW OF PHYSIOLOGY, VOL 76 (2014)

Review Medicine, General & Internal

Cardiovascular remodelling in coronary artery disease and heart failure

Gerd Heusch et al.

LANCET (2014)

Review Cardiac & Cardiovascular Systems

Cardiac Metabolism and its Interactions With Contraction, Growth, and Survival of Cardiomyocytes

Stephen C. Kolwicz et al.

CIRCULATION RESEARCH (2013)

Article Medicine, Research & Experimental

Look Back in Anger - What Clinical Studies Tell Us About Preclinical Work

Thomas Hartung

ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION (2013)

Review Cardiac & Cardiovascular Systems

Muscle oxygen transport and utilization in heart failure: implications for exercise (in)tolerance

David C. Poole et al.

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY (2012)

Article Biotechnology & Applied Microbiology

384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids

Amy Y. Hsiao et al.

BIOTECHNOLOGY AND BIOENGINEERING (2012)

Article Cell Biology

The H9C2 cell line and primary neonatal cardiomyocyte cells show similar hypertrophic responses in vitro

Sarah J. Watkins et al.

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2011)

Review Cardiac & Cardiovascular Systems

Methods in cardiomyocyte isolation, culture, and gene transfer

William E. Louch et al.

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2011)

Review Cell Biology

From 3D cell culture to organs-on-chips

Dongeun Huh et al.

TRENDS IN CELL BIOLOGY (2011)

Article Biochemistry & Molecular Biology

Essential role of TRPC1 channels in cardiomyoblasts hypertrophy mediated by 5-HT2A serotonin receptors

Cecile Vindis et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2010)

Article Cardiac & Cardiovascular Systems

Cell Communications in the Heart

Daniela Tirziu et al.

CIRCULATION (2010)

Article Biochemistry & Molecular Biology

Myocyte stress 1 plays an important role in cellular hypertrophy and protection against apoptosis

Andrea L. Koekemoer et al.

FEBS LETTERS (2009)

Article Cardiac & Cardiovascular Systems

Ser9 phosphorylation of mitochondrial GSK-3β is a primary mechanism of cardiomyocyte protection by erythropoietin against oxidant-induced apoptosis

Katsuhiko Ohori et al.

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY (2008)

Article Developmental Biology

PI3K/Akt and CREB regulate adult neural hippocampal progenitor proliferation and differentiation

Joseph Peltier et al.

DEVELOPMENTAL NEUROBIOLOGY (2007)

Article Biochemistry & Molecular Biology

ZAK re-programs atrial natriuretic factor expression and induces hypertrophic growth in H9c2 cardiomyoblast cells

CY Huang et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2004)