Comprehensive characterization of Dactylorhin and loroglossin in Dactylorhiza hatagirea D. DON using ultra-performance liquid chromatography-mass spectrometry

Dactylorhiza hatagirea D. Don is acclaimed for different medicinal properties but a little is known about its secondary-metabolites. The purpose of this study was to reveal the components of D. hatagirea by UPLC- ESI/TQ-MS approach. Metabolite profiling of D.hatagirea is reported for the first time for rapid identification of bioactive components. Molecular mass of twelve compounds were determined from LC-MS analysis of the different frac-tions and structures of five compounds were elucidated with MS/MS fragmentation pattern analysis. Dactylorhin C (2), B (3), A (5), and Loroglossin (4) were derivatives of 2,3-dihydroxy-2-(2-methylpropyl)butanedioic acid and 2-hydroxy-2-(2-methylpropyl)butanedioic acid. In addition, Sucrose (1) was also identified. The obtained results confirmed the presence of Dactylorhins, Loroglossin and Sucrose which have already been isolated from the plant.

Automated summary

The components of D. hatagirea were analyzed using UPLC-ESI/TQ-MS approach for the first time. Twelve compounds were identified and the structures of five compounds were elucidated through MS/MS fragmentation pattern analysis. The presence of Dactylorhins, Loroglossin, and Sucrose in the plant was confirmed.