Journal
CHEMICAL COMMUNICATIONS
Volume 51, Issue 51, Pages 10310-10313Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc03746h
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Funding
- National Natural Science Foundation of China [31100587]
- National Institute of Health [R01 GM085267, R01 HD065122]
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Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a D-galactosyl-beta 1-3-N-acetyl-D-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an alpha 2-3-sialyltransferase or an alpha 2-6-sialyltransferase.
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