Optimization of exopolysaccharide produced by L. kefiranofaciens ZW3 using response surface Methodology

The primary objective of this study was to optimize the production of exopolysaccharide (EPS) produced by L. kefiranofaciens ZW3 through the utilization of response surface methodology (RSM). Central Composite Design (CCD) was used along with three variables to study the response variable. The effects of lactose (0.16-1.84%), tryptophan (0.16-0.84%), and pH (5.06-7.24) on EPS production were studied. RSM analysis revealed that coefficient of determination (0.9907) was good for the second-order polynomial model. The optimum values of explanatory variables were 0.36% lactose, 0.23% tryptophan, and pH value of 5.19. Additional experiments were performed to confirm the adequacy of this model. The predicted value of EPS at optimized level was 1609 mg/L, while its experimental value at optimized level was 1602 & PLUSMN; 3 mg/L. This study could help to produce higher amount of EPS from L. kefiranofaciens ZW3, which has a lot of industrial applications.

Automated summary

The primary objective of this study was to optimize the production of exopolysaccharide (EPS) produced by L. kefiranofaciens ZW3 using response surface methodology (RSM). The effects of lactose, tryptophan, and pH on EPS production were studied using Central Composite Design (CCD). RSM analysis showed a good fit for the second-order polynomial model, with lactose, tryptophan, and pH values of 0.36%, 0.23%, and 5.19, respectively, being the optimal conditions. The study confirms the adequacy of the model and suggests that it could help enhance EPS production for various industrial applications.