Journal
MICROCHIMICA ACTA
Volume 184, Issue 3, Pages 715-724Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-016-2028-8
Keywords
Detection; Immunoassay; Monoclonal antibody; Foodborne pathogen; Listeriosis; L. ivanovii; Transmission electron microscopy; Gold nanoparticles; Milk analysis
Categories
Funding
- National Natural Science Foundation of China [21471068]
- MOST
- MOE [2014BAD04B10, 201513006, 2016YFF0202300]
- Natural Science Foundation of Jiangsu Province [BK201501, BK20140003, BE2013613, BE2013611]
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A lateral flow assay for rapid, simple and efficient determination of L. monocytogenes is presented. A monoclonal antibody (mAb) 1C1 against the peptide from P60 protein of L. monocytogenes was prepared and labeled with gold nanoparticles (AuNPs). The mAb 1C1 was paired with the mAb 10E7 against the P60 protein of all the Listeria spp. and used as a capture bioligand in a lateral flow assay. The AuNP-based strip test can detect the supernatant of eight common L. monocytogenes serotypes including 1/2a, 1/2b, and 4b with an equivalent detection limit of 3.7 x 10(6) CFUai...mL(-1) but does not detect four other Listeria spp. (L. ivanovii, L. innocua, L. welshimeri, and L. grayi). There was no cross-reactivity with six other Gram-negative and Gram-positive bacteria. The method was applied to the quantification of L. monocytogenes species in spiked milk samples within 13 h.
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