Journal
MICROCHIMICA ACTA
Volume 183, Issue 10, Pages 2831-2836Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-016-1935-z
Keywords
Nanomaterial; Template synthesis; Fluorescence; Quenching; Serum analysis
Categories
Funding
- National Key Basic Research Program of China [2014CB744502]
- Special Foundation for State Major Basic Research Program of China [2015FY111100]
- National Natural Science Foundation of China [21575165]
- Natural Science Foundation of Hunan province [2015JJ1019]
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Copper nanoclusters (Cu-NCs) were prepared by reducing CuCl2 with ascorbic acid in the presence of the short peptide template Cys-Cys-Cys-Asp-Leu. They were characterized by UV-vis absorption and fluorescence spectroscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. The Cu-NCs have a size of similar to 2 nm, can be well dispersed in water and are photostable. Their fluorescence (peaking at 425 nm under 365-nm excitation) is quenched by Fe(III) ions. Based on this finding, a sensitive and selective fluorescence assay for the detection of Fe(III) was developed. Under optimized conditions and a pH value of 2.0, the assay displays a linear response in the 0.05 to 30 mu M Fe(III) concentration range, with a detection limit of 20 nM based on an S/N ratio of 3. The assay was successfully applied to the determination of Fe(III) in spiked human serum where is gave recoveries that ranged from 96.2 % to 98.3 %.
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