Fabrication of chitosan and Trianthema portulacastrum mediated copper oxide nanoparticles: Antimicrobial potential against MDR bacteria and biological efficacy for antioxidant, antidiabetic and photocatalytic activities

Biopolymer based metal oxide nanoparticles, prepared by eco-friendly approach, are gaining interest owing to their wide range of applications. In this study, aqueous extract of Trianthema portulacastrum was used for the green synthesis of chitosan base copper oxide (CH-CuO) nanoparticles. The nanoparticles were characterized through UV-Vis Spectrophotometry, SEM, TEM, FTIR and XRD analysis. These techniques provided evidence for the successful synthesis of the nanoparticles, having poly-dispersed spherical shaped morphology with average crystallite size of 17.37 nm. The antibacterial activity for the CH-CuO nanoparticles was determined against multi-drug resistant (MDR), Escherichia coli, Pseudomonas aeruginosa (gram-negative), Enterococcus faecium and Staphylococcus aureus (gram-positive). Maximum activity was obtained against Escherichia coli (24 & PLUSMN; 1.99 mm) while least activity was observed against Staphylococcus aureus (17 & PLUSMN; 1.54 mm). In-vitro analysis for biofilm inhibition, EPS and cell surface hydrophobicity showed >60 % inhibitions for all the bacterial isolates. Antioxidant and photocatalytic assays for the nanoparticles showed significant activities of radical scavenging (81 & PLUSMN; 4.32 %) and dye degradation (88 %), respectively. Antidiabetic activity for the nanoparticles, determined by invitro analysis of alpha amylase inhibition, showed enzyme inhibition of 47 & PLUSMN; 3.29 %. The study signifies the potential of CH-CuO nanoparticle as an effective antimicrobial agent against MDR bacteria along with the antidiabetic and photocatalytic activities.

Automated summary

Biopolymer based metal oxide nanoparticles, prepared by an eco-friendly approach using aqueous extract of Trianthema portulacastrum, showed successful synthesis of poly-dispersed spherical nanoparticles with an average crystallite size of 17.37 nm. The chitosan base copper oxide (CH-CuO) nanoparticles exhibited strong antibacterial activity against MDR bacteria, including Escherichia coli and Staphylococcus aureus, and showed inhibitory effects on biofilm formation, EPS production, and cell surface hydrophobicity. Additionally, the nanoparticles demonstrated antioxidant activity, photocatalytic activity, and antidiabetic activity through alpha amylase inhibition.