Journal
MICROCHIMICA ACTA
Volume 184, Issue 2, Pages 547-555Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-016-2046-6
Keywords
Magnetic mesoporous carbon; Ferric oxide; Zirconia; Nanoparticles; Hydrophilic carbon material; Metal oxide affinity chromatography; Phosphopeptide; Enrichment; Human serum; MALDI-TOF MS
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Funding
- State Key Program of National Natural Science of China [21235005]
- National Key Scientific Instrument and Equipment Development Project [2012YQ120044]
- National Natural Science Foundation of China [21475044]
- model Project of the Research and Application of the Common Key Technology about Chemical Reagent of Basis Scientific Research [2015BAK44B00]
- foundation of Shanghai Research Institute of Criminal Science and Technology
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Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles were synthesized from resol, ZrO(NO3)(2), ferric acetylacetonate, and triblock copolymer F127. The method involves a multi-component co-assembly strategy associated with direct carbonization. The resulting carbon material is shown to be useful as a metal oxide affinity chromatography (MOAC) material for enrichment of phosphopeptides owing to its large mesoporous (4.8 nm) surface area (442 m(2) g(-1)), large pore volume (0.37 cm(3) g(-1)) and excellent hydrophilicity. The metallic iron and ferric oxide particles modified on the mesoporous carbon exert a magnetic force and, in combination with the metallic zirconia, is a viable MOAC material for enrichment of low-abundance phosphopeptides. Because of metal chelation between metallic nanoparticles and the phosphate groups of phosphopeptides, the zirconia/magnetic mesoporous carbon displays high selectivity even at a phosphopeptide/nonphosphopeptide molar ratio of 1:500. As little as 1.5 fmol of phosphopeptides become detectable. The MOAC was successfully applied to the identification by MALDI-TOF MS of phosphopeptides in human serum and nonfat milk.
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