Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 248, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2023.125879
Keywords
Curculigo orchioides; Polysaccharide; MC3T3-E1 cells; Osteogenic differentiation; BMP/Smad pathway; Wnt/beta-catenin pathway
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A new heteropolysaccharide COP70-1 was purified from the crude polysaccharide CO70 isolated from Curculigo orchioides, which can promote the osteoblastic differentiation of MC3T3-E1 cells by activating the BMP/Smad and Wnt/beta-catenin pathways. It has the potential to treat ovariectomy-induced osteoporosis in rats.
The crude polysaccharide CO70 isolated from Curculigo orchioides could alleviate ovariectomy-induced osteoporosis in rats. To clarify the bioactive components, a new heteropolysaccharide (COP70-1) was purified from CO70 in this study, which was consisted of beta-D-Manp-(1 ->, -> 4)-alpha-D-Glcp-(1 ->, -> 4)-beta-D-Manp-(1 ->, -> 3,4)-beta-D-Manp-(1 ->, -> 4,6)-beta-D-Manp-(1 ->, and -> 4,6)-alpha-D-Galp-(1 ->. COP70-1 significantly promoted the osteoblastic differentiation of MC3T3-E1 cells through improving alkaline phosphatase activity, the deposition of calcium as well as up-regulating the expression of osteogenic markers (RUNX2, OSX, BSP, OCN, and OPN). Furthermore, COP70-1 stimulated the expression of critical transcription factors of the BMP and Wnt pathways, including BMP2, p-SMAD1, active-beta-catenin, p-GSK-3 beta, and LEF-1. In addition, LDN (BMP pathway inhibitor) and DKK-1 (Wnt pathway inhibitor) suppressed the COP70-1-induced osteogenic differentiation of MC3T3-E1 cells. Therefore, COP70-1 was one of the bioactive constituents of C. orchioides for targeting osteoblasts to treat osteoporosis by triggering BMP/Smad and Wnt/beta-catenin pathways.
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