Journal
MICROCHIMICA ACTA
Volume 183, Issue 11, Pages 2917-2923Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-016-1903-7
Keywords
Nanomaterial; Salt-induced aggregation; Visual test; Diagnostic test; Tear analysis; Saliva analysis
Categories
Funding
- National Natural Science Foundation of China [21305004]
- Joint Fund for Fostering Talents of National Natural Science Foundation of China and Henan Province [U1304205]
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The authors describe an aptasensor for visual and fluorescent detection of lysozyme via an inner filter effect (IFE). The assay is based on the fact that red gold nanoparticles (AuNPs) act as powerful absorbers of the green fluorescence of CdTe because of spectral overlap. If the lysozyme-binding aptamer is adsorbed onto the surface of the AuNPs, the salt-induced aggregation of AuNPs (that leads to a color change from red to blue) does not occur and the IFE remains efficient. If lysozyme is present, it will bind the aptamer and thereby prevent its adsorption on the AuNPs. As a result, the salt-triggered aggregation of the AuNPs will occur. Consequently, color will change from red to blue, and green fluorescence will pop up because the IFE is suppressed. Under optimum conditions, fluorescence is linearly related to lysozyme concentration in the 1.0 nM to 20 nM concentration range, with a 0.55 nM limit of detection. The method is perceived to be of wider applicability in that it may be used to design other visual and fluorescent assays if appropriate aptamers are available.
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