Journal
MICROCHIMICA ACTA
Volume 183, Issue 3, Pages 1107-1114Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-015-1686-2
Keywords
Magnetic particles; MEMS; Gold film; Lithography; Sputtering
Categories
Funding
- National Natural Science Foundation of China [61273065]
- National Science and Technology Support Program [2012BAK08B05]
- National Key Laboratory Research Fund [9140C790403110C7905]
- Natural Science Foundation of Shanghai [13ZR1420800]
- Support fund of Joint research center for advanced aerospace technology of Shanghai Academy of Spaceflight Technology-Shanghai Jiao Tong University
- Analytical and Testing Center in Shanghai Jiao Tong University
- Center for Advanced Electronic Materials and Devices in Shanghai Jiao Tong University
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We report on a giant magnetoresistance (GMR) based immunosensor for the carcinoembryonic antigen (CEA) labeled with superparamagnetic microparticles (Dynabeads). The GMR sensor contains 200 GMR strips and was fabricated by micro-electromechanical system (MEMS) technology. This system can detect Dynabeads in concentrations down to 10 nga <...mL(-1). Sandwich immunoassays were employed on the surface of a gold film modified with a self-assembled monolayer and using biotinylated secondary antibody against CEA and streptavidinylated Dynabeads. With DC magnetic fields in the range of 40 to 90 Oe, CEA can be detected with a detection limit as low as 10 pga <...mL(-1). Samples spiked with different concentrations of CEA can be distinguished clearly, and the method is deemed to be well suited for clinical use.
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