4.7 Article

A poly(ADP-ribose) polymerase-1 activity assay based on the FRET between a cationic conjugated polymer and supercharged green fluorescent protein

Journal

CHEMICAL COMMUNICATIONS
Volume 51, Issue 76, Pages 14389-14392

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc04170h

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Funding

  1. National Natural Science Foundation of China [21222507, 21305037, 21475037, 21235002]
  2. National Basic Research Program of China (973 Program) [2011CB911002]
  3. Foundation for Innovative Research Groups of NSFC [21221003]
  4. Excellent Youth Foundation of Hunan Scientific Committee [2015JJ1005]

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A label-free and convenient strategy for PARP-1 activity assay and inhibitors assessment has been developed based on the fluorescence resonance energy transfer (FRET) between a cationic conjugated polymer (CCP) and supercharged green fluorescent protein (scGFP).

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