The B-box transcription factor PabBBX27 in the regulation of chlorophyll biosynthesis and photosynthesis in poplar (Populus alba x P. Berolinensis)

Chlorophyll is responsible for absorbing light which is required to convert solar energy into chemical energy during photosynthesis. Despite remarkable progresses achieved in chlorophyll biosynthesis, the relevant mech-anisms in poplar remain unclear. Here, we identified a BBX gene, PabBBX27, which was from Populus alba x P. Berolinensis, may regulate the biosynthesis of chlorophyll in poplar. PabBBX27 transcripts were most abundant in young leaves of poplar. Transgenic lines overexpressing (OE) PabBBX27were characterized by dark green leaf. The contents of chlorophyll and photosynthates in the OE lines were significantly increased. In addition, photosynthetic rate, stomatal guidance, intercellular CO2 concentration, transpiration rate, initial fluorescence F0, maximum light efficiency (Fv/Fm), actual photochemical quantum yield and photochemical quenching of the OE lines were all increased. Genes relate to chlorophyll metabolic pathway and photosynthesis were activated in the OE lines as well. Yeast one-hybrid results showed that PabBBX27 may enhance their activities by binding to the G-box elements in the promoter sequences of these genes. Taken together, our results provide a new insight for the molecular mechanism of chlorophyll metabolic pathway and the cultivation of new varieties with high photosynthetic utilization efficiency for poplar.

Automated summary

We identified a BBX gene, PabBBX27, in poplar, which may regulate the biosynthesis of chlorophyll and improve photosynthetic efficiency. Overexpression of PabBBX27 resulted in increased chlorophyll and photosynthates content, higher photosynthetic rate and efficiency, as well as activation of genes related to chlorophyll metabolic pathway and photosynthesis. Our findings provide new insights into the molecular mechanism of chlorophyll metabolic pathway and the cultivation of new varieties with high photosynthetic utilization efficiency in poplar.