Journal
MICROBIOLOGICAL RESEARCH
Volume 182, Issue -, Pages 49-58Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2015.09.004
Keywords
Microbulbifer sp; Alginate lyase; Characterization; Alginate oligosaccharides; Antioxidant activity
Categories
Funding
- National Natural Science Foundation of China [31401632]
- Xiamen Southern Ocean Technology Center of China [13PZP002NF03, 13GZP004NF10]
- Open Research Foundation of Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, China [M20130905]
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A novel alginate-degrading marine bacterium Microbulbifer sp. ALW1 was isolated from rotten brown alga. An extracellular alginate lyase was purified to electrophoretic homogeneity and had a molecular mass of about 26.0 kDa determined by SDS-PAGE and size exclusion chromatography. This enzyme showed activities towards both polyguluronate and polymannuronate indicating its bifunctionality while with preference for the former substrate. Using sodium alginate as a substrate, strain ALW1 alginate lyase was optimally active at 45 degrees C and pH 7.0. It was stable at 25 degrees C, 30 degrees C, 35 degrees C and 40 degrees C, but not stable at 50 degrees C. This alginate lyase showed good stability over a broad pH range (5.0-9.0). The enzyme activity was increased to 5.1 times by adding NaCl to a final concentration of 0.5 M. Strain ALW1 alginate lyase produced disaccharide (majority) and trisaccharide from alginate indicating that this enzyme could be a good tool for preparation of alginate oligosaccharides with low degree of polymerization (DP). The alginate oligosaccharides displayed the scavenging abilities towards radicals (DPPH, ABTS(+) and hydroxyl) and the reducing power. Therefore, the hydrolysates exhibited the antioxidant activity and had potential as a natural antioxidant. (c) 2015 Elsevier GmbH. All rights reserved.
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