Journal
METHODS
Volume 100, Issue -, Pages 3-15Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.02.017
Keywords
Electron microscopy
Funding
- Wellcome Trust [096685/Z/11/Z]
- Wellcome Trust [096685/Z/11/Z] Funding Source: Wellcome Trust
- MRC [G1000567] Funding Source: UKRI
- Medical Research Council [G1000567] Funding Source: researchfish
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Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins >150 kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freezing. In the last few years, cryo-EM has undergone a 'resolution revolution', owing to both advances in imaging hardware, image processing software, and improvements in sample preparation, leading to growing number of researchers using cryo-EM as a research tool. However, cryo-EM is still a rapidly growing field, with unique challenges. Here, we summarise considerations for imaging of a range of specimens from macro molecular complexes to cells using EM. (C) 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.orgilicensesiby/4.0/).
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