Journal
METHODS
Volume 96, Issue -, Pages 69-74Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2015.10.005
Keywords
BAC transgenesis; Recombineering; Protein localization; Genome engineering; Protein tagging
Funding
- Max Planck Society
- German Federal Ministry of Education and Research (BMBF) [031A099]
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The localization of a protein is intrinsically linked to its role in the structural and functional organization of the cell. Advances in transgenic technology have streamlined the use of protein localization as a function discovery tool. Here we review the use of large genomic DNA constructs such as bacterial artificial chromosomes as a transgenic platform for systematic tag-based protein function exploration. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license
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