Journal
METHODS
Volume 104, Issue -, Pages 48-54Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.01.008
Keywords
MALDI-TOF MS; Antibiotic resistance; Antibiotic susceptibility testing; Semi-quantitative analysis
Funding
- Bayerische Forschungsstiftung (Forschungsverbund ForBIMed-Biomarker in der Infektionsmedizin)
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The increasing resistance to antibiotics is an urgent health care problem. Detection of resistant microorganisms is the pre-requisite for initiating an adequate therapy and implementing respective hygiene measures. Depending on the species and the method employed for analysis, the time to result of antibiotic resistance testing ranges between five and 24 h. As MALDI-TOF MS has become an established tool for the fast species identification in microbiological laboratories a time gap between the results of species identification and the information about antibiotic susceptibility arises. Here, we present a semi quantitative MALDI-TOF MS-based approach for the detection of resistance in different species against different antibiotics. (C) 2016 Elsevier Inc. All rights reserved.
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