Journal
METHODS
Volume 103, Issue -, Pages 57-67Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.02.004
Keywords
Single-molecule; FRET; RNA structure; Fluorescence; 3D model
Funding
- NASA Postdoctoral Program Fellowship
- Cambridge Biomedical Research Centre
- MRC [G0801709, G0800142] Funding Source: UKRI
- Medical Research Council [G0800142, G0801709] Funding Source: researchfish
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The importance of elucidating the three dimensional structures of RNA molecules is becoming increasingly clear. However, traditional protein structural techniques such as NMR and X-ray crystallography have several important drawbacks when probing long RNA molecules. Single molecule Forster resonance energy transfer (smFRET) has emerged as a useful alternative as it allows native sequences to be probed in physiological conditions and allows multiple conformations to be probed simultaneously. This review serves to describe the method of generating a three dimensional RNA structure from smFRET data from the biochemical probing of the secondary structure to the computational refinement of the final model. (C) 2016 Elsevier Inc. All rights reserved.
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